Detection of siderophores on the wings of bats with white-nose syndrome via molecular networking.

<p>Molecular networking of the MS/MS data was used to determine the structural relationships between the metabolites detected from wing surfaces, cultured <i>Pseudogymnoascus destructans</i>, and commercial siderophore standards. The siderophores desferrichrome (<i>m/z</i> 710.324) and ferrichrome (<i>m/z</i> 763.230) were observed from the wings of bats with white-nose syndrome and from cultured <i>P</i>. <i>destructans</i> and formed consensus nodes with commercial standards (A,B). Triacetylfusarinine C (<i>m/z</i> 928.344) was also present on WNS wings and in the <i>P</i>. <i>destructans</i> colony (C). None of the three siderophores were detected on the wings of healthy bats. In A and C insets only the node with the <i>m/z</i> of interest and its first and second neighbors have been displayed from the overall cluster for simplicity.