Dramatic chromosomal rearrangements in 3D7Δ<i>Sir2a</i> gene disruption mutants.

<p>A: Southern blots of genomic DNA from 3D7 and 3D7Δ<i>Sir2a</i>, digested with Taq1 and probed for the first sub-telomeric repeat, TARE1, or the conserved ‘ATS’ region of <i>var</i> genes. Arrows indicate bands that differ between the two lines. B: Pulsed-field gel electrophoresis showing altered karyotype in 3D7Δ<i>Sir2a</i> compared to 3D7. C: Comparative genomic hybridization plots of 3D7 and 3D7Δ<i>Sir2a</i>. Only those chromosomes containing significant CNVs are shown. The log₂ratio of Cy3/Cy5 value is plotted against chromosomal position. D: Chromosomal rearrangements in 3D7Δ<i>Sir2a</i> detected by array CGH. The location of each <i>var</i> gene is shown in orange in the schematic chromosomes. The two locations that show CNV in NF54 are indicated with arrows. Deletions and amplifications are highlighted with boxes.