Summary of Paramyxovirinae matrix protein nuclear trafficking phenotypes.

<p>^(-),<0%;-, 0–10%;</p><p>^+/-, 10–25%;</p><p>⁺, 25–50%;</p><p>⁺⁺, 50–75%;</p><p>⁺⁺⁺, 75–100%;</p><p>⁺⁺⁺⁺,>100%;</p><p>IP-IB, immunoprecipitation followed by immunoblot;</p><p>MIP, maximum intensity projection of 3D confocal micrographs;</p><p>BiFC, bimolecular fluorescence complementation; XYZ, XYZ planes view of 3D confocal micrographs;</p><p>N.D., not determined;</p><p>*, NES mutation gave unexpected increased nuclear exclusion rather than the expected increased nuclear localization (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004739#ppat.1004739.g002" target="_blank">Fig. 2H</a> for quantification);</p><p>**, Subcellular localization phenotypes of the NES and the various NLSbp2 mutants for NDV-M and MeV-M are not congruent (compare <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004739#ppat.1004739.s003" target="_blank">S3 Fig</a>. and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004739#ppat.1004739.g002" target="_blank">Fig. 2F and 2G</a>);</p><p>***, Recombinant MuV rescued with NES or NLSbp2 mutations. Defects in viral morphogenesis were observed but subcellular localization of MuV-M could not be determined due to a lack of specific anti-MuV-M antibodies.</p><p>Summary of Paramyxovirinae matrix protein nuclear trafficking phenotypes.