Nucleolar localization of Sendai M during live virus infection and perturbation of ubiquitination.

<p>HeLa cells were infected with WT rSeV-eGFP at MOI 10. At 18h post infection, cells were treated with 50 μM MG132/0.5% DMSO or 0.5% DMSO for 8h. <b>(A)</b> Wide field epifluorescence of cells counterstained with anti-SeV-M antibodies, red, and with DAPI to visualize nuclei, blue. <b>(B)</b> XYZ Planes View of 3D confocal micrographs. Cells were counterstained with DAPI to visualize nuclei, blue, anti-SeV-M antibodies, red, and anti-Fibrillarin antibodies to visualize nucleoli, grayscale. Relative nucleolar localization of SeV-M was determined by its localization to Fibrillarin signal. Scale bars 10 μm. ****p<0.0001 by a Student’s t test.