Substrate specificities of TlProR, FaProR, HjProR, CdProR, and AbHypE.

<p>The reaction mixture (1 ml) consisted of 10 mM of substrate and each purified enzyme as follows in Tris-HCl buffer (pH 8.0): TlProR, 1.3 μg; FaProR, 100 μg; HjProR, 570 μg; CdProR, 3.6 μg; AbHypE, 4.9 μg. L-A2C is L-azetidine-2-carboxylate. Samples at the indicated times were analyzed by HPLC (means ± S.D., <i>n</i> = 3). Values on the bars of TlProR indicate specific activity (unit/mg protein), which are similar to those in Tables <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120349#pone.0120349.t001" target="_blank">1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120349#pone.0120349.t002" target="_blank">2</a>.