Total protease activity (units x 10³/ml) secreted by <i>P</i>. <i>destructans</i> grown in static liquid cultures.

<p>Various protein-supplemented minimal nutrient broth media and complex culture media were used. Controls are total activity accumulated in culture medium, and residual activity was determined following treatment with the protease inhibitors EDTA, PMSF, and E-64. Minimal nutrient broth with K, keratin, G, gelatin, E, elastin, C, casein. Complex media: TSB, tryptic soy broth, TP, tryptone peptone, BHI, brain-heart infusion, PP, proteose peptone. (Mean and standard error for triplicate samples.)