The contents of the autophagic proteins in the hypothalamus from diet induced obese mice with 8 and 16 weeks of treatment.

<p><b>A.</b> Western-blotting shows the Beclin-1 to β-actin ratio in the hypothalamus of obese mice with 8 weeks on a HFD. <b>B.</b> Western-blotting shows LC3-II to β-actin ratio in the hypothalamus of obese mice with 8 weeks on a HFD. <b>C.</b> p62 content in the hypothalamus of obese mice with 8 weeks on a HFD. <b>D.</b> Beclin-1 content in the hypothalamus of obese mice with 16 weeks on a HFD. <b>E.</b> LC3-II to β-actin ratio in the hypothalamus of obese mice with 16 weeks on a HFD. <b>F.</b> p62 content in the hypothalamus of obese mice with 16 weeks on a HFD. Values are shown as mean ± SEM. *P < 0.05; n = 4–7 animals per group.