Western Blot analyses of Nrf2 signaling in kidneys of normal control mice, vehicle-treated mice with anti-GBM-GN, and EGCG-treated mice with anti-GBM-GN.

<p>Western blot showed significant reduction in the levels of Nrf2, GCLc, GCLm, and GPx1 in vehicle-treated mice with anti-GMB-GN, compared to the levels in normal control mice. EGCG treatment of mice with anti-GBM-GN led to significant increase in the levels of Nrf2, GCLc, GCLm, and GPx1. In contrast, vehicle-treated mice with anti-GMB-GN showed significantly increased levels of NQO1 and HO1, compared to the levels in normal control mice. EGCG treatment further increased the NQO1 levels but did not significantly affect the HO1 levels, compared to the levels in vehicle-treated mice. Values are expressed as mean ± SEM; n = 8–10 in each group.