Immunohistochemistry for inflammatory cells in kidneys of normal control mice, vehicle-treated mice with anti-GBM-GN, and EGCG-treated mice with anti-GBM-GN.

<p>Kidneys from mice 4 weeks after induction of anti-GBM-GN with or without EGCG treatment and kidneys from normal control mice were evaluated for inflammatory cell infiltration. Immunohistochemical analyses showed markedly increased interstitial infiltration of lymphocytes and macrophages in the vehicle-treated group (original magnification x 400). In contrast, the EGCG-treated animals exhibited significantly less interstitial infiltration of lymphocytes and macrophages. No significant inflammatory cell infiltrates were observed in the normal control mice. The number of lymphocyte and macrophages in glomeruli and interstitial compartment are depicted in the bar graphs. Values are mean ± SEM; n = 8–10 in each group.