A demonstration that this system can be used to assess the effects of monoclonal antibodies (mAbs) upon the process of aggregate formation and coalescence.

<p>Preparations of MB-435- α6HG6 cells were continuously infused with either of two anti-integrin mAbs. A. Untreated control preparation of aggregates (blue) undergoing coalescence between 338 and 426 hours. In this period, eight of the nine aggregates coalesced. B. A preparation of aggregates (blue) treated with the anti-ß-1 integrin mAb, AIIB2, underwent growth but did not coalesce. C. A preparation of aggregates (blue) treated with the anti-α-3 integrin mAB, P1B5, underwent accelerated coalescence, and exhibited a massive increase in the formation of probe cells (red), many of which exited the aggregates.