Ectopic expression of EOMES in the ectoderm of D*V and D*C embryonic chicken comb tissue.

<p>A-D: Immunohistochemical labeling did not detect any expression of EOMES in either the mesenchyme or ectoderm of the <i>D*N</i> developing comb region. E-K: EOMES is expressed in the ectoderm of both <i>D*V</i> and <i>D*C</i> embryos as early as embryonic day 7 (E7) and continuing through E12/E15. All genotypes showed similar patterns of expression of EOMES in the brain, which was used as a positive control for the antibody specificity. By E12 and E15 a change in tissue morphology is readily apparent in both <i>D*V</i> and <i>D*C</i> embryos. ect; ectoderm, mes; dermal mesenchyme. Scale bar in K is 200 μm and applies to A-C, E-G, I and J. Bars in D and H are 100 μm and L is 1 mm.