KM11073 enhanced BMP-2-induced osteoblast differentiation in C2C12 cells.

<p>Cell viability was assayed 1 and 3 days after treatment with KM11073 (<i>A</i>). Effect of KM11073 on BMP-2-stimulated ALP induction. Cells (4 × 10³ cells/well) were cultured in a 96-well plate for 1 day and then the medium replaced with DMEM containing 5% FBS and KM11073 in the presence or absence of rhBMP-2 (100 ng/ml). The medium was changed every 3 days. On differentiation day 6, ALP staining and its activity were assayed <i>(B)</i>. Effect of noggin on KM11073-mediated enhancement of BMP-2-stimulated ALP induction. Osteogenesis was enhanced by KM11073 in the presence of BMP-2 on differentiation days 0 and 2, and then noggin was treated on differentiation day 4. On differentiation day 6, ALP staining and its activity were assayed (<i>C</i>). *** <i>p</i> < 0.001 compared to the BMP-2-treated group; ^##<i>p</i> < 0.01, ^###<i>p</i> < 0.001 compared to the group treated with BMP-2 and KM11073.