Vaginal challenge of AcHERV-triHPV-immunized mice with PVs.

<p>Mice were administered AcHERV-triHPV or PBS (control) three times over a 2-week interval by the SL or IM route. (A-C) Mice sublingually administered PBS. (D-L) Mice sublingually administered AcHERV-triHPV at a dose of 1×10⁸ copies (D-F), 1×10⁹ copies (G-I), or 1×10¹⁰ copies (J-L). (M-O) Mice intramuscularly immunized with 1×10⁹ copies. Eight weeks after the first immunization, mice received vaginal challenge with luciferase-expressing PV16 (A,D,G,J,M), PV18 (B,E,H,K,N), or PV58 (C,F,I,L,O). Three days after the challenge, mice were anesthetized and injected with luciferin, and the levels of luminescence were detected with an IVIS 200 bioluminescence imaging system.