JSI-124 inhibits VEGF-induced migration, invasion and tubular structure formation of endothelial cells.

<p>(A) Effect of JSI-124 on VEGF-induced HUVECs proliferation determined by CCK-8 assay. HUVECs (5×10³/ per well) were starved with 0.1% FBS medium and then treated with or without VEGF (20 ng/mL) and different concentrations of JSI-124 for 24 h. (B) JSI-124 remarkably inhibited VEGF-induced endothelial cells migration measured by transwell migration assay. HUVECs were seeded in the upper chamber of transwell (coated no matrigel) and treated with different concentrations of JSI-124. The bottom chamber was filled with ECM supplemented with VEGF. After about 8 h, the migrated HUVECs passed through the membrane were quantified. (C) JSI-124 strongly suppressed VEGF-induced endothelial cells invasion measured by transwell invasion assay. HUVECs were seeded in the upper chamber of transwell (coated with 50% matrigel) and treated with different concentrations of JSI-124. The bottom chamber was filled with ECM supplemented with VEGF. After about 8 h, the migrated HUVECs passed through the membrane were quantified. (D) JSI-124 inhibits VEGF-induced tube formation of HUVECs determined by capillary-like tube formation assay. Cells (4×10⁴/ well) were placed in the 96-well plates coated with matrigel. After 4 h of incubation, tubular structure formation was captured under microscope. All data were expressed as mean ± SD. * <i>P</i> < 0.05; ** <i>P</i> < 0.01 versus VEGF alone.