JSI-124 inhibites the U87MG cells–induced migration of HUVECs.

<p>U87MG cells were cultured in the well (lower chamber). Cells were then treated with or without JSI-124 for 8 hours. After treatment, the medium from the lower chamber was replaced with fresh DMEM without JSI-124, and the insert containing a monolayer of HUVECs in DMEM was then placed into the well. After 8 hours, migrated cells were photographed and counted. (A) Schematic diagram of coculture assay. (B) Control. HUVECs were seeded onto the upper chamber, whereas the bottom chamber contains only DMEM without U87MG cells. (C) HUVECs cocultured with U87MG cells untreated with JSI-124. (D) HUVECs cocultured with U87MG cells pretreated with 100 nM. All data were expressed as mean ± SD. * <i>P</i> < 0.05, versus control.