Effect of temperature on the activity and stability of the purified laccase.

<p>(a) Optimal temperature (open circles) was determined at pH 7.5 by using guaiacol as the substrate. Residual activity (closed circles) was determined after incubation at 40°C–90°C for 1 h. (b) Residual activity was determined after incubation at 70°C, 80°C, and 90°C, respectively, for 0–4 h. The graphs display the average values from triplicate measurements. The error bars represent the standard deviation.