SDS-PAGE, zymogram analyses and glycosylation analyses of the purified LacTT.

<p>(a) The gel stained with Coomassie Brilliant Blue R-250. Lane M: protein marker; Lane 1: purified LacTT after heat denaturation; Lane 2: purified LacTT without heat denaturation. (b) The zymogram stained with 0.1 mM SGZ and 2 mM guaiacol. Lane M: protein marker; Lane 1: unheated purified LacTT stained with 0.1 mM SGZ; Lane 2: unheated purified LacTT stained with 2 mM guaiacol; Lane 3: heat-denatured LacTT stained with 0.1 mM SGZ; Lane 4: heat-denatured LacTT stained with 2 mM guaiacol. (c) Deglycosylation analyses of the purified laccase. Lane 1: purified LacTT; Lane 2: purified LacTT was deglycosylated by PNGase F; Lane M: protein marker.