Over-expression of CCR4 enhanced the invasion of human GC cells.

<p>SGC-7901 and BGC-823 cells were transiently transfected with empty (MOCK) or CCR4-expressing vector (EGFP-CCR4) using lipofectamine2000, and the transfection efficiency was confirmed by a fluorescence microscope (A), RT-PCR (B) and FCM (C) at 24 or 48 hours after transfection, respectively. D, cells were resuspended in 200μl serum-free RPMI-1640 with a concentration of 1.5×10⁵/ml after transfection at 24h, and then allowed to invade the Transwell inserts (8-μm pores) coated with Matrigel for another 24h. The cells that invaded through the inserts were counted and photographed under a light microscopy at 200× magnification. The data are presented as representative images or as the mean ± SD (n = 3 in each column) from more than three individual experiments. Gray-filled histogram, CCR4; empty histogram, isotype. Data are presented as representative images and mean ± SD and for three separate experiments from each group. *, <i>P</i>< 0.05; **, <i>P</i>< 0.01.