Whole Genome Maps using different restriction enzymes show putative tandem duplication of chromosomal regions.

<p>A: Top four panels of maps generated using <i>Bam</i>HI, <i>Nhe</i>I, <i>Kpn</i>I and <i>Afl</i>III respectively show the duplicated region in <i>V</i>. <i>cholerae</i> 1154–74 (O49). B: The bottom three panels of maps are that of 10432–62 (O27) using <i>Bam</i>HI, <i>Nhe</i>I and <i>Afl</i>III. In both cases, the location of duplication was found to be around 1240 kb to 1506 kb on reference M66–2 Chr I. The duplicated genome segments are indicated by the orange box in the <i>in silico</i> map of the reference strain. In some panels both assemblies with the single and two copies (resulting from re-analyses of the single molecule data from this region) are shown. The duplicated copies where they could be resolved are indicated by the red circles. The exact lengths of the duplications in the two strains cannot be unequivocally determined by this WGM data. It is also possible that the maps represent a mixed population of cells containing single and two copies of the duplication. (Scale bar 100 Kb).