<p>Quantitative PCR analyses in the renal tissue of rats treated for 28 days (in fold change): angiotensinogen (A), renin (B), ACE (C), ET-1 (D), TNF-α (E) and collagen type III (F) from sham (n = 6), losartan (n = 6), leptin (n = 8) and leptin plus losartan (n = 6) treated rats. *p<0.05 <i>versus</i> the sham and <sup>#</sup>p<0.05 <i>versus</i> the leptin-treated rats.</p
<p>A total of 10 samples for each group were used, and each sample was run in triplicate for real-ti...
<p>n = 4 rats/per group, values are mean±S.E. **P<0.01 when compared to the control group.</p
<p>(<b>A</b>) representative RT-PCR images for Wnt1, LRP5, β-catenin, RANKL, RANK and β-actin expres...
<p>Agt (Angiotensinogen); ACE (Angiotensin Converting Enzyme); TNF-α (Tumor Necrosis Factor), TGF-β ...
<p>According to the comparative method (RQ = 2<sup>–ΔΔCt)</sup>, the expression level of each gene w...
<p>The renal morphology of the leptin and/or losartan-treated rats. A representative image of the gl...
<p>Individual samples were normalized to β-actin mRNA levels and data are expressed as the mRNA expr...
<p>Total RNA and protein were extracted from the kidney respectively. Total RNA was subjected to rea...
<p>*p<0.001 vs. sham, <sup>†</sup>p<0.05 vs. sham, <sup>‡</sup>p<0.05 vs. SNx.</p
<p>Saline, rats treated with Saline; Lf, rats treated with lactoferrin; PDC, rats treated with potas...
<p>Eight genes were chosen for quantitative RT-PCR (qRT-PCR). Each bar represents one of four fold-d...
<p>Real-time RT-PCR for profibrogenic genes. Data are means±SEM.</p><p>Mann-Whitney test: *<i>P</i><...
The role of hyperleptinemia in cardiovascular diseases is well known; however, in the renal tissue, ...
The role of hyperleptinemia in cardiovascular diseases is well known; however, in the renal tissue, ...
<p>(A) RT-PCR for 18S RNA in culture medium (CM) from BMSC controls and culture medium treated with ...
<p>A total of 10 samples for each group were used, and each sample was run in triplicate for real-ti...
<p>n = 4 rats/per group, values are mean±S.E. **P<0.01 when compared to the control group.</p
<p>(<b>A</b>) representative RT-PCR images for Wnt1, LRP5, β-catenin, RANKL, RANK and β-actin expres...
<p>Agt (Angiotensinogen); ACE (Angiotensin Converting Enzyme); TNF-α (Tumor Necrosis Factor), TGF-β ...
<p>According to the comparative method (RQ = 2<sup>–ΔΔCt)</sup>, the expression level of each gene w...
<p>The renal morphology of the leptin and/or losartan-treated rats. A representative image of the gl...
<p>Individual samples were normalized to β-actin mRNA levels and data are expressed as the mRNA expr...
<p>Total RNA and protein were extracted from the kidney respectively. Total RNA was subjected to rea...
<p>*p<0.001 vs. sham, <sup>†</sup>p<0.05 vs. sham, <sup>‡</sup>p<0.05 vs. SNx.</p
<p>Saline, rats treated with Saline; Lf, rats treated with lactoferrin; PDC, rats treated with potas...
<p>Eight genes were chosen for quantitative RT-PCR (qRT-PCR). Each bar represents one of four fold-d...
<p>Real-time RT-PCR for profibrogenic genes. Data are means±SEM.</p><p>Mann-Whitney test: *<i>P</i><...
The role of hyperleptinemia in cardiovascular diseases is well known; however, in the renal tissue, ...
The role of hyperleptinemia in cardiovascular diseases is well known; however, in the renal tissue, ...
<p>(A) RT-PCR for 18S RNA in culture medium (CM) from BMSC controls and culture medium treated with ...
<p>A total of 10 samples for each group were used, and each sample was run in triplicate for real-ti...
<p>n = 4 rats/per group, values are mean±S.E. **P<0.01 when compared to the control group.</p
<p>(<b>A</b>) representative RT-PCR images for Wnt1, LRP5, β-catenin, RANKL, RANK and β-actin expres...