The function of <i>tyrB</i> and <i>aspC</i> in pigmentation in <i>A</i>. <i>media</i> strain WS.

<p>(A) Wild-type <i>A</i>. <i>media</i> strain WS, WSΔ<i>tyrB</i>, WSΔ<i>aspC</i>, WSΔ<i>tyrB</i>Δ<i>aspC</i>, WSΔ<i>tyrB</i> (pBBR1MCS-5), WSΔ<i>tyrB</i> (pBBR1MCS-5-<i>tyrB</i>), WSΔ<i>tyrB</i>Δ<i>aspC</i> (pBBR1MCS-5), WSΔ<i>tyrB</i>Δ<i>aspC</i> (pBBR1MCS-5-<i>tyrB</i>), WSΔ<i>tyrB</i>Δ<i>aspC</i> (pBBR1MCS-5-<i>aspC</i>) were cultured in LB, and then at 72 h and 96h post-inoculation, the OD₄₀₀ of the cultures were determined. (B) Photographs of cultures from 72 h LB cultures of the strains. (C) Pigment production can be restored to WSΔ<i>tyrB</i> and WSΔ<i>tyrB</i>Δ<i>aspC</i> by the addition of 5 mM 4-hydroxyphenylpyruvate to the LB.