EIF5A2 Expression in GC cells and tissues and validation of the intervention.

<p>(<b>A</b>) <i>EIF5A2</i> mRNA expression was examined by real-time quantitative RT-PCR with <i>GAPDH</i> serving as a loading control. (<b>B</b>) Protein expression in cell lines was confirmed by western blot analysis with GAPDH as a loading control. (<b>C</b>) EIF5A2 protein in human GC tissues was higher than that of the paired distant non-tumor tissues. (D) All three EIF5A2-siRNAs, especially #1, efficiently repressed <i>EIF5A2</i> mRNA expression in HGC27 cells. (E) Western blotting reveals that EIF5A2 was knocked down by the treatment of EIF5A2- siRNAs in HGC27 cells. (<b>F</b>) <i>EIF5A2</i> mRNA was significantly upregulated by transient transfection of EIF5A2 plasmids in MKN45 cells. (<b>G</b>) EIF5A2 protein was significantly upregulated by transient transfection of EIF5A2 plasmids in MKN45 cells. (H and I) T2-siRNA could efficiently suppress MTA1 expression in HGC27 cells.