MiR-153 Overexpression repressed the p-SMAD2, p-SMAD3, EGFR and IGFBP-3 expression.

<p>The MG-63 was treated in serum-free medium in the presence and absence of TGF-β (50 ng/ml), scramble, or miRNA-153 mimic for 24 h. (A) Expression of p-SMAD2, t-SMAD2, p-SMAD3 and t-SMAD3 was detected using western blotting. GAPDH was used as a loading control. (B) Expression of EGFR and IGFBP-3 was detected using western blotting. GAPDH was used as a loading control. (C) The mRNA expression of EGFR was detected using qRT-PCR. The expression of MMP9 was normalized to GAPDH. (D) The mRNA Expression of IGFBP-3 was detected using qRT-PCR. The expression of EGFR was normalized to GAPDH.