Hypoxia inducible factor expression.

<p><b>A</b>: Expression of HIF-1α target genes, characteristic for stabilization of HIF-1α, 3 and 7 days after single DMOG injection <b>B</b>. Representative images of HIF-1α staining, showing HIF stabilization in all DEN groups, mostly located in and around cholangioma and HCC lesions and near portal areas. <b>C</b>. mRNA expression for HIF-1α markers. For animals sacrificed after 22 weeks; saline Ctrl: n = 8, PBS Ctrl: n = 6, Early DMOG: n = 8, Int DMOG: n = 6 and for animals sacrificed after 27 weeks; saline Ctrl: n = 8, PBS Ctrl: n = 7, Adv DMOG: n = 7. Scale bars: 200μm, *: p<0,05; **p<0,01 Hif-1α: hypoxia inducible factor 1 alpha, DMOG: dimethyloxaloylglycine, DEN: diethylnitrosamine.