Changes in relative miRNA arm expression upon infection.

<p>(A) Detection and relative expression of miRNA hairpin arms. Stacked bar plot shows the proportion of detected miRNA hairpins for which either one or both mature miRNA sequences were detected. Density plot shows the distribution of log₂ expression ratios for hairpins where both arms were expressed. (B) An example of one miRNA, miR-361, which showed a strong infection-dependent change in relative arm expression. Axes show the expression of the 3p and 5p arms of all sequenced individuals at each time point. In each panel, colours denote the different time points, and infection conditions are plotted in separate panels. The displacement of points towards the right of the plot in infected samples at later time points shows that the change in the expression ratio of the two arms is due to the increased expression of the 3p arm that, in some conditions, becomes dominant.