PERK activation stimulated medulloblastoma cells to produce VEGF-A.

<p>(A) Real-time PCR analysis showed that 0.01 nM AP20187 treatment significantly increased the expression of CHOP, GADD34, and VEGF-A in Fv2E-PERK1 cells, but did not affect the expression of VEGFR2 and BIP. (B) ELISA analysis showed that 0.01 nM AP20187 treatment significantly increased the production of VEGF-A in Fv2E-PERK1 cells. (C) Real-time PCR analysis showed that 10 μM Salubrinal treatment significantly increased the expression of CHOP, GADD34, and VEGF-A in Daoy cells, but did not affect the expression of VEGFR2 and BIP. (D) ELISA analysis showed that 10 μM Salubrinal treatment significantly increased the production of VEGF-A in Daoy cells. (E) Real-time PCR analysis showed that 10 μM Salubrinal treatment significantly increased the expression of CHOP, GADD34, and VEGF-A in UW228 cells, but did not affect the expression of VEGFR2 and BIP. (F) ELISA analysis showed that 10 μM Salubrinal treatment significantly increased the production of VEGF-A in UW228 cells. The experiments were repeated at least three times. Error bars represent SD, *<i>P</i> < 0.05.