Intensity of GTTR fluorescence in the LSC cristae over time.

<p>(A) The intensity of diffuse GTTR fluorescence in dark cells (DC) at 0.5 hours significantly increased over time to a peak value at 3 hours before declining. Diffuse GTTR fluorescence in transitional cells (TC), hair cells (HC), and supporting cells (SC) also increased in a similar manner, peaking at 3 hours before declining at 4 hours. (B) Comparison of diffuse cytoplasmic GTTR fluorescence in the different cell types of the LSC using one way ANOVA with a post hoc test revealed significantly increased intensity of diffuse GTTR fluorescence in transitional cells compared to dark cells, hair cells, and supporting cells (p<0.01) at 0.5 hour. At 1 hour, diffuse GTTR fluorescence in transitional cells continued to be significantly higher than in hair cells and supporting cells. At later time-points (2, 3, and 4 hours), no significant difference was found in the diffuse GTTR fluorescence of dark cells, transitional cells, hair cells, and supporting cells (p>0.05). (C) The intensity of punctate GTTR fluorescence in dark cells, transitional cells, and supporting cells at 0.5 hours significantly increased over time to a peak at 3 hours, and did not decline significantly at 4 hours. (D) Comparison of GTTR puncta intensity in the different cell types of the LSC using one way ANOVA with a post hoc test revealed significantly increased intensity of GTTR puncta in transitional cells compared to supporting cells (p<0.05) at 0.5 hours. At 1 hour, GTTR puncta in transitional cells and dark cells were significantly more intense than in supporting cells (p<0.01). At 2 hours, only GTTR puncta in transitional cells were significantly more intense compared to supporting cells (p<0.01). At 3 and 4 hours, puncta GTTR fluorescence in dark cells, transitional cells, and supporting cells was not significantly different (p>0.05). (For A-D: * p<0.05, ** p<0.01, ***p<0.001; mean ± s.d.; n = 5).