Knockdown of CoREST1 reduced MDA-MB-231 tumor formation.

<p>MDA-MB-231 cells were transduced with lentivirus encoding a control (shCtrl) or either of 2 shRNA constructs targeting CoREST1 (shCoR). <b>(A)</b> CoREST1 expression in MDA-MB-231 cell lines was quantified using RT-qPCR relative to β-actin expression. Differences were determined using Student’s t-test (n = 6 experiments; mean±s.e.m.). <b>(B)</b> Representative image of CoREST1 expression measured by immunoblotting (n>3 experiments). <b>(C)</b> NOD/SCID females were injected with shCoR #1 or shCtrl cells into the fourth mammary glands. Masses greater than 3 mm in diameter were defined as tumors (*p<0.001, Fischer’s exact test). <b>(D)</b> Tumor growth curve from mice injected with shCoR #1 cells compared to shCtrl controls (*p<0.005, Mann-Whitney test). <b>(E)</b> At end stage, tumor weights were measured from mice injected with either shCtrl or shCoR #1 cells. Differences were determined by Mann-Whitney test.