Antagonism of CD317/tetherin-induced NF-kB signaling.

<p><b>A</b>) CD317/tetherin-induced NF-κB activation by Vpu alleles. Luciferase activity of the NF-κB reporter was determined 40 h post transfection of 293T cells with expression plasmids for CD317/tetherin or filler, the indicated VpuIRESGFP expression plasmids and luciferase reporter plasmids. HIV-1 NL4.3 Vpu and HIV-1 M WITO Vpu served as controls. Mean values of 6–9 transfections are shown with the indicated standard deviation. <b>B</b>) Effect of EC and CP Vpu alleles on CD317/tetherin-induced NF-κB activation. Statistical significance was assessed using two-tailed Mann-Whitney U-Test (p = 0.29), bars represent median and interquartile ranges. <b>C</b>) Correlation of Vpu-mediated inhibition of CD317/tetherin-induced NF-κB activity and release of infectious virions. Statistical analyses were done using Spearman’s correlation.