α-Catulin knockdown enhances susceptibility of melanoma cells to cisplatin.

<p>(<b>A-F</b>) Stable infected (n.s., sh-catu2) (<b>A</b>) Mel.7, (<b>B</b>) Mel.17, (<b>C</b>) Mel.15, (<b>D</b>) Mel.7 spheroids, (<b>E</b>) Mel.7 (n.s., sh-catu1) or (<b>F</b>) Melanocytes (mock, myc-α-catulin) were treated with different concentrations of cisplatin for 48h and cell survival normalized to untreated cells (pos. contr.). Viability was analyzed by CellTiter-Blue Assay. (<b>G</b>) Stable infected Mel.7 spheroids were treated with 200 μg/ml cisplatin for 96 hours and diameter of the spheroids determined before and after treatment and statistically evaluated. Observations (•) mean (-) n = 14, (<b>H</b>) Microscopic images from Mel.7 spheroids.