Western blot analysis and quantification of RALDH2 in postnatal human ocular tissues.

<p><b>(A)</b> Cytosol fractions from ocular tissues of donors aged 16–46 years were immunblotted with anti-RALDH2 or anti-RPE65. <i>Top Panel</i>: RALDH2 immunopositive bands (∼ 55–59 kDa) were present in the choroid (C), sclera (S), retina (R), and RPE (E) of postnatal human eyes. <i>Bottom Panel</i>: anti-RPE65 was used to identify the presence of RPE contamination in the retina, choroid, and sclera samples. 3 μg total protein/lane was loaded for each blot. <b>(B)</b> Relative abundance of RALDH2 was measured as the integrated optical density (IOD) per 3 μg total protein of RALDH2-immunopositive bands. <b>(C)</b> Average RALDH2 abundance (± s.e.m.) in ocular tissues of all donors presented in (B) (n = 5). *<i>p</i> < 0.05, **<i>p</i> < 0.01 (one-way ANOVA followed by Tukey-Kramer test for multiple comparisons).