Aptamer inhibition of the binding of pro-uPA to LRP and of the binding of pro-uPA:uPAR complexes to vitronectin.

<p>(<b>A</b>) SPR sensorgram showing the binding of samples containing 25 nM pro-uPA to LRP immobilized on the sensor surface. The pro-uPA was pre-incubated with control RNA (black), upanap-12 (blue), or upanap-126 (red), as indicated. Solid line (150 nM RNA), broken line (50 nM RNA) and dotted line (17 nM) RNA. No significant effect was observed for 200 nM control RNA relative to no addition (data not shown). (<b>B</b>) The number of RU bound to LRP on the sensor surface with 25 nM pro-uPA and varying aptamer concentrations was normalized to the amount of RU bound in the absence of aptamer and plotted versus the aptamer concentration. (<b>C</b>) SPR sensorgram showing the binding of samples of 10 nM pro-uPA:uPAR complex to monomeric vitronectin immobilized on the sensor surface. The pro-uPA:uPAR was pre-incubated with RNA as indicated. (<b>D</b>) The number of RU bound to vitronectin on the sensor surface with 10 nM pro-uPA:uPAR complex and varying aptamer concentrations was normalized to the amount of RU bound in the absence of aptamer and plotted versus the aptamer concentration. No significant effect was observed with 200 nM control RNA (omitted in the figure). Data represent the average of three replicates.