Expression of BDNF and receptor TrkB on progenitor cells.

<p>(A) Immunoblot data depicted higher expression of BDNF in freshly isolated Cyc cells in comparison to Wt. Bar graph indicates relative protein abundance of BDNF normalized to ß-actin control quantified by densitometry (n = 3; mean ± SD; *<i>p</i><0.05; t-test). (B) mRNA intensity signals of TrkB receptor determined by microarray analysis (n = 2; mean ± SD). (C-D) Immunofluorescence micrographs display the expression of TrkB receptor on Sca-1 cells. Nuclei were stained with DAPI. (Scale bar: 50μm, image magnification: 40x). (E) Protein abundance of TrkB receptor on Sca-1 cells quantified against ß-actin control by densitometry (n = 3, mean ± SD). Representative immunoblot of both full length and truncated forms of TrkB receptor on Wt and Cyc cells is shown below.