Half-live of N1303K with and without treatment with compounds.

<p>HEK 293 cell lines stably expressing either mutant were treated with cycloheximide (25μg/ml) and harvested after 1, 2, 4, 8, or 24 h. (<b>A, C)</b> The half-life of the C band of the N130K mutant is much shorter than the B band, which has a surprisingly long half-life. When C3+C4 5 (μM) was applied, the levels of both the B and C bands were higher at each time point for (<b>B, D)</b> N1303K. The major effect of the corrector combination was a prolongation of the half-life of the C band of N1303K. Cells were cultured for 16 hours at 37°C with or without correctors. Data are expressed as the mean ± SD of 3 independent experiments.