BC-15 binds to various kinds of human cancer cells.

<p>Cells were stained with FITC-labelled BC-15 aptamer (Upper row) or FITC-labelled random control aptamer (Middle row, green). All the cells were counter stained with 0.25% Evans blue for 10 min to reveal whole-cell morphology (red). Lower row show CK immunofluorescence staining of different types of cancer cells. These cultured cells were trypsinized, dropped onto the coated glass slides, followed by staining with Alexa 594 labelled anti-CK18 antibody, and counter stained with DAPI. PL45, pancreatic adenocarcinoma cell; MCF-7, breast cancer cell; A549, lung carcinoma cell; MDA-MB-231, breast cancer cell; HT-29, colon adenocarcinoma cell; MCF10A, non-tumourigenic immortalised mammary epithelial cells. Scale bar, 20μm.