Neutrophil infiltration and ICAM-1 expression in kidney 24 hr after LPS challenge.

<p>(A) Immunolocalization of neutrophils (indicated by arrows) in mice kidneys using anti-NIMP-R14 antibody (Abcam). Magnification: 40x (scale bar, 100 μm). (B) Neutrophil infiltration was assessed by measuring renal MPO activity as previously described [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120728#pone.0120728.ref037" target="_blank">37</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120728#pone.0120728.ref038" target="_blank">38</a>]. Data are expressed as mean ± SEM (N = 3 mice per group), *<i>P</i><0.05. Real time RT-PCR (C) and Western blot (D) analysis of ICAM-1 expression in renal tissues after LPS challenge. Western blot analysis was performed using anti-ICAM-1 and anti-β-actin antibody as described. Densitometric analysis of relative ICAM-1 expression after normalization with β-actin is shown in the lower panel. Data are expressed as the mean ± SEM (N = 5–6 mice per group), *<i>P</i><0.05.