Disruption of IFN-β autocrine-paracrine pathways with neutralizing antibody.

<p>Bm1.11 cells were infected with 10 IFU/ cell <i>C</i>. <i>muridarum</i> to measure the amount of IFN-β secreted into the supernatants for 24 hours by ELISA. The supernatants were supplemented at each time-point listed with either 0.1μg/ml of: <b>(A)</b> IFN-β neutralizing antibody, or <b>(B)</b> isotype control antibody. The amount of IFN-β secreted into the supernatants <u>after</u> the addition of antibody (ab/IgG-24h) was estimated by subtracting out the amount of IFN-β secreted in control experiments in which supernatants were instead collected and assayed for IFN-β synthesis at the same time-point (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119235#sec002" target="_blank">Materials and Methods</a>). <i>Results are representative data from one of three independent experiments</i>. * = <i>p</i>< 0.05 <i>compared to 24h C</i>. <i>muridarum infection control</i>; <u><i>0-ab/IgG</i></u><i>denotes “0hr PI until time antibody added”;</i><u><i>ab/IgG-24h</i></u><i>denotes “time antibody added until 24h PI”</i>.