Restored <i>TOPgal</i> activity and cranial nerve development in <i>Hhat</i>^{<i>Creface/Creface</i>}; <i>Ptch1</i>^{<i>Wig/Wig</i>} double mutants.

<p>(A-C) <i>TOPgal</i> expression in E9.5 controls (A), <i>Ptch1</i>^{<i>Wig/Wig</i>} mutant (B), and <i>Hhat</i>^{<i>Creface/Creface</i>};<i>Ptch1</i>^{<i>Wig/Wig</i>} compound mutant (C) embryos. Red arrowheads indicate trigeminal nerve region and white arrowheads show the facial nerve region. (D-F) Whole mount <i>Sox10 in situ</i> hybridization in the indicated control or mutant embryos. (C and F) <i>Hhat</i>^{<i>Creface/Creface</i>};<i>Ptch1</i>^{<i>Wig/Wig</i>} double mutants showed a restoration of cranial nerve patterning indicated by <i>TOPgal</i> and <i>Sox10</i> staining levels similar to controls (A and D). Scale bars: 200μm.