Hemolytic activity of cells and supernatants on human and sheep blood.

<p>Bacteria were cultivated in LB medium with and without 0.04% bile bovine. Cultures [A] and [C] and filtered supernatants [B] and [D] were added to a suspension of each blood type and incubated for four hours. Red blood cell lysis was determined at OD₅₄₀. Control strains were VN-0022 (<i>tdh/trh</i> negative) and ATCC439996 (<i>tdh</i> positive, <i>trh</i> negative). All experiments were performed twice.