Effect of RNAse III treatment on mAb 3G1 and 2G4 binding.

<p>Immunofluorescence assay performed on Vero cell monolayers infected with WNV_KUNV at MOI: 10 or mock infected and untreated (-RNAse III) or treated with dsRNA-specific nuclease RNAse III (+RNAse III). Shown are cells stained with mAbs 3G1 or 2G4, anti-dsRNA mAb J2 or WNV_KUNV NS1-specific mAb 3.1112G. All antibodies are labelled with goat anti-mouse Alexafluor 488 (green). Nuclei are stained with Hoechst nuclear stain (blue). Images were taken at 40x magnification. Scale bar denotes 10 μm.