Membrane integrity analysis in DM1 patient biopsies.

<p>(A) Calcium staining using Alizarin Red S. No or only few calcium-positive fibers (arrowheads), indicative of abnormal elevated calcium level, were found in DM1 and control biopsies. Calcium deposits were abundant in the DMD sample. DMD muscle sections showed fatty tissue infiltration (asterisks) and fibrosis (stars), which were also detected in some of the DM1 patient biopsies. Scale bars indicate 100 μm. Results from only one of the three controls and three of the six examined DM1 patients are shown. All data are summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121556#pone.0121556.t002" target="_blank">Table 2</a>. (B) Dystrophin immunostaining. In contrast to the DMD patient biopsy, expression and localization of dystrophin in DM1 and control quadriceps muscles were normal. Spectrin staining was included as a cell membrane reference. Scale bars indicate 100 μm. (C) Quantification of dystrophin expression. Data is represented as mean intensity per fiber (n>50). Error bars indicate 95% confidence interval (CI). Data are summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121556#pone.0121556.t002" target="_blank">Table 2</a>.