Characterization of the inhibitory mechanism of GPC-N114 on CVB3 3D<sup>pol</sup>.
- Lonneke van der Linden (550170)
- Laia Vives-Adrián (709988)
- Barbara Selisko (249846)
- Cristina Ferrer-Orta (243160)
- Xinran Liu (365526)
- Kjerstin Lanke (264378)
- Rachel Ulferts (709989)
- Armando M. De Palma (709990)
- Federica Tanchis (709991)
- Nesya Goris (709992)
- David Lefebvre (72593)
- Kris De Clercq (366776)
- Pieter Leyssen (709993)
- Céline Lacroix (622858)
- Gerhard Pürstinger (709994)
- Bruno Coutard (219756)
- Bruno Canard (219785)
- David D. Boehr (709995)
- Jamie J. Arnold (120696)
- Craig E. Cameron (120731)
- Nuria Verdaguer (5649955)
- Johan Neyts (55640)
- Frank J. M. van Kuppeveld (17308)
Publication date
March 2015
DOIAbstract
<p>(A) GPC-N114 does not compete with UTP. The experiment was performed for CVB3 3D<sup>pol</sup> as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004733#ppat.1004733.g002" target="_blank">Fig. 2E</a> for EMCV 3D<sup>pol</sup>. (B) GPC-N114 is more efficient when added to the reaction mix before the template-primer. Order-of-addition experiments were performed by measuring CVB3 3D<sup>pol</sup> elongation activity when GPC-N114 was added to the reaction mix before or after poly(rA)/dT15. A typical of three independent experiments is presented.</p
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