Peptide specific HLA-E restricted CD8⁺ T-cell clones have an effector memory phenotype.

<p>T cell clones were cultured in the absence of peptide specific stimulation and RNA was isolated from T-cell clones, RNA expression profiles were determined using dcRT-MLPA and data were normalized for GAPDH expression within each sample (A-C). A. RNA expression levels of classical cellular subset and memory markers of T-cell clones; B. RNA expression levels of cytotoxic effector function associated molecules; C. RNA expression levels of lineage associated transcription factors. D. Flow cytometric analysis of T-cell phenotype, T cell clones were directly stained from culture, a representative T-cell clone is shown (D6-2B4). Gating strategy in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004671#ppat.1004671.s001" target="_blank">S1 Fig.</a> E. Flow cytometric analysis of effector molecules, T-cell clones activated with αCD3/28 beads for 24 hours followed by intracellular staining, a representative T-cell clone is shown (D6-2B4). F. Flow cytometric analysis of lineage determining transcription factors, T-cell clones were directly stained from culture using intracellular staining protocols. Dashed lines represent transcription factor staining in PBMCs, grey (D2–1B9) and black are examples of different T-cell clones (D2-4A1, D6-1F11, D2-2A9).