T cell-intrinsic increase in PD-1 KO CD4+ TEM-phenotype cells.

<p>Cells from thymi, spleens and lymph nodes were analyzed by flow cytometry for donor-derived WT (GFP^-DsRed^-) and PD-1 KO (GFP⁺DsRed^-) CD4⁺ cells 8 weeks after bone marrow reconstitution in lethally irradiated DsRed hosts. A, Dot plots show donor-derived WT (GFP^-DsRed^-) and PD-1 KO (GFP⁺DsRed-) thymocytes. On thymocytes the expression of CD4 and CD8 was analyzed (left). The distribution WT GFP^- and GFP⁺ PD-1 KO cells were assessed among CD4 SP cells (right). Numbers indicate percentages in each region. Column represents the average value of PD-1 KO/WT CD4 SP thymocyte ratios with error bar indicating SD. In one of the thymus experiments the WT donor cells were GFP⁺ and PD-1 KO cells were GFP^-. B, Donor-derived WT (GFP^-DsRed^-) and PD-1 KO (GFP⁺DsRed^-) CD4⁺ T cells from spleens and mesenteric lymph nodes were further analyzed for expression of CD44 and CD62L. Numbers indicate percentages in each region. C, Graph indicates the average percentage of cell subsets among WT- and PD-1 KO-derived CD4⁺ cells in spleens, as in B. Error bars denote SD. D, Total numbers of WT- and PD-1 KO-derived CD4⁺ cell subsets in spleens with error bars indicating SD. Data are representative of 2 individual experiments, n = 6.