The AR-EGFP transgene increases testis weights in rescue mice.

<p>A) Agarose gel analysis is shown of PCR reactions used to genotype mice employed primers that identify the AR-EGFP transgene (tg), the Cre recombinase (Cre) or floxed endogenous AR (AR flox) and the AR transgene (AR tg). Mouse strains were identified that expressed no transgenes (wild type, wt), the Cre recombinase (Cre), a floxed AR gene (AR flox) as well as SCARKO and rescue mice. Mice expressing the AR-EGFP transgene (tg), the transgene + Cre (Tg + CRE) or the transgene in the presence of a floxed AR gene (Tg + AR flox) also were identified. The sizes of DNA markers in base pairs (bp) are shown below each gel image. B) Testis weight/body weights are provided for mice expressing the transgenes as described in A. C) The testis weight/body weight for the individual rescue mouse strains are shown relative to the same wt and SCARKO values used in panel B. Values with different lowercase letters differ significantly (P<0.05). Panel B, n = 6 to 39, panel C, n = 3 to 14).