The response of α7^G, α7^E260A:G heterozygote and α7^E260A:G homozygote mice to i.n. LPS challenge.

<p>Mice (α7^G, heterozygote α7^E260A+/-:G or homozygote α7^E260A+/+:G) were challenged with i.n. LPS (250 μg/mouse in 30 μl Saline) or saline (30 μl per mouse). For all experiments the results are shown as scatter plots and quantitated with bar graphs reflecting the results of three independent experiments (4 to 5 mice per experimental group). Error bars represent standard error of the mean (SEM) and statistical significance was determined using the Student’s t-test. (A) a small sample of blood (50 μl) was collected 24 hours LPS post-challenge and assessed for CD11b⁺Gr1⁺ cells (left panel). On day 3 after challenge, mice were sacrificed and BALF and interstitial cells isolated as described above and in the Methods for determination of total number of nucleated cells (A, middle and right panels). (B) BALF cells and (C) interstitial CD45⁺ cells isolated at 72 hrs post-challenge from α7^G, α7^E260A+/-:G, α7^E260A+/+:G. (D) BALF and interstitial CD45⁺ cells are shown and further identified by markers for monocyte/macrophages (Ly6C) and PMN (Ly6G).