<p>The expression of the neural marker <i>neurod</i> was analyzed by WISH at 24 hpf in control and <i>slc7a6os</i> morphants. In MO injected embryos no expression of <i>neurod</i> was detected in anterior and posterior ganglia while a down-regulation was observed in dorsal diencephalon, telencephalon and octave statoacustic ganglia (B, flat-mounted embryos). The altered expression pattern of <i>neurod</i> seen in morphants embryos is rescued by the expression of synthetic <i>slc7a6os</i> mRNA. Abbreviations: ad/av/f, anterodorsal/anteroventral lateral line/ facial placodes/ ganglia; dd, dorsal diencephalon; o, octaval/statoacustic ganglia; p, posterior lateral line ganglia; t, telencephalon.</p
<p><b>(A,C,E,G,I)</b> Embryos at 60 hpf treated with 1% DMSO from 24 hpf were used as negative contr...
<p>Utilizing the <i>Neurod1:LacZ</i> knock-in allele (<i>Neurod1<sup>LacZ/+</sup></i>) and immunoflu...
<p>ISH analysis showed that <i>Gsh2</i>, <i>Mash1</i> and <i>Dlx2</i> genes are all expanded dorsall...
<p>Control and <i>slc7a6os</i> morphant embryos were analyzed by WISH for <i>pax2a</i> gene expressi...
<p>At 48 hpf, lateral view show that MO injected embryos have an evident pericardial and yolk-sac ed...
<p>Embryos were injected at the two-cells stage with 6 ng/embryo of <i>slc7a6os</i>-MOspl1 splicing-...
<p><b>(A–L)</b><i>In situ</i> hybridization of neuronal marker genes with wild-type (A,C,E,G,I,K), a...
<p>Dorsal views of 3-somite-stage wild-type (A, B, C, D) and <i>arl6ip1</i> knockdown embryos (E, F,...
<p>(A-D) Whole mount hybridization in situ of embryos injected unilaterally with 90 ng SPC7 morpholi...
<p>At 72 hpf, the pericardial and yolk-sac edema, CNS malformations and the general morphology alter...
<p>Dorsal view (rostal towards the top) of confocal fluorescent composite images of hindbrain branch...
<p><b>(A, B)</b> Embryos are under lateral view. <b>(A)</b> Expressions of brain regionalization gen...
<p>Lateral views (A–F) of 72 hpf embryos processed for anti-Hu immunofluorescence assaying (IFA) to ...
<p>(A) Knockdown of APPb disrupts the projections of axons Vp and VII (3 dpf) when injected with APP...
NeuroD, a basic-helix-loop-helix transcription factor found in humans, regulates neuronal differenti...
<p><b>(A,C,E,G,I)</b> Embryos at 60 hpf treated with 1% DMSO from 24 hpf were used as negative contr...
<p>Utilizing the <i>Neurod1:LacZ</i> knock-in allele (<i>Neurod1<sup>LacZ/+</sup></i>) and immunoflu...
<p>ISH analysis showed that <i>Gsh2</i>, <i>Mash1</i> and <i>Dlx2</i> genes are all expanded dorsall...
<p>Control and <i>slc7a6os</i> morphant embryos were analyzed by WISH for <i>pax2a</i> gene expressi...
<p>At 48 hpf, lateral view show that MO injected embryos have an evident pericardial and yolk-sac ed...
<p>Embryos were injected at the two-cells stage with 6 ng/embryo of <i>slc7a6os</i>-MOspl1 splicing-...
<p><b>(A–L)</b><i>In situ</i> hybridization of neuronal marker genes with wild-type (A,C,E,G,I,K), a...
<p>Dorsal views of 3-somite-stage wild-type (A, B, C, D) and <i>arl6ip1</i> knockdown embryos (E, F,...
<p>(A-D) Whole mount hybridization in situ of embryos injected unilaterally with 90 ng SPC7 morpholi...
<p>At 72 hpf, the pericardial and yolk-sac edema, CNS malformations and the general morphology alter...
<p>Dorsal view (rostal towards the top) of confocal fluorescent composite images of hindbrain branch...
<p><b>(A, B)</b> Embryos are under lateral view. <b>(A)</b> Expressions of brain regionalization gen...
<p>Lateral views (A–F) of 72 hpf embryos processed for anti-Hu immunofluorescence assaying (IFA) to ...
<p>(A) Knockdown of APPb disrupts the projections of axons Vp and VII (3 dpf) when injected with APP...
NeuroD, a basic-helix-loop-helix transcription factor found in humans, regulates neuronal differenti...
<p><b>(A,C,E,G,I)</b> Embryos at 60 hpf treated with 1% DMSO from 24 hpf were used as negative contr...
<p>Utilizing the <i>Neurod1:LacZ</i> knock-in allele (<i>Neurod1<sup>LacZ/+</sup></i>) and immunoflu...
<p>ISH analysis showed that <i>Gsh2</i>, <i>Mash1</i> and <i>Dlx2</i> genes are all expanded dorsall...