Morphological analysis of <i>slc7a6os</i> morphants and phenotypic rescue at 48 h.

<p>At 48 hpf, lateral view show that MO injected embryos have an evident pericardial and yolk-sac edema and the somite structures are not well-defined (D-E) when compared to controls (A, D). Dorsal view of morphants shows that the CNS abnormalities are more pronounced and reveals a severe disorganization of the following structures: midbrain–hindbrain boundary, dorsal diencephalon and tegmentum (F). Also at this developmental stage the expression of synthetic <i>slc7a6os</i> mRNA rescues the wild type phenotype in morphants (G-I). Abbreviations: so, somites; teg, tegmentum; dd, dorsal diencephalon; MHB, midbrain–hindbrain boundary.