Profile of endogenous and TuMV-derived siRNAs in plants expressing HA-AGO2_DAD in an <i>ago2–1</i> background.

<p>Values are average and SE from two biological replicates normalized to reads per million. Inoculated rosette leaf and systemically infected cauline leaf samples were collected at 7 and 15 dpi, respectively. Inflorescence samples were collected at 10 dpi. (A) Panel I: number of reads by size, class, and polarity, for TuMV-derived siRNAs in input and HA-AGO2_DAD IP. Panel II: for 21 and 22 nt TuMV-derived siRNAs, enrichment in HA-AGO2_DAD IP. Enrichment is defined as immunoprecipitate (IP) reads/ input reads, expressed on a log₂ scale. Panel III: proportion (in percentage) of 5’ nt in 21 nt and 22 nt TuMV-derived siRNAs by fraction. Numbers were rounded to the nearest integer. (B) and (C) TuMV genome-wide distribution of 21 nt TuMV-derived siRNAs in input (B) and HA-AGO2_DAD IP (C). Panel I: TuMV-infected inflorescence. Panel II: TuMV-inoculated rosette leaves. Panel III: rosette leaves inoculated with TuMV-AS9. Panel IV: cauline leaves systemically infected with TuMV-AS9. Reads were plotted for each 1 nt position. The scale was capped at 150 reads.