Rapamycin inhibited mitochondrial mTOR and blocked radiation-induced bioenergetics switching.

<p>(A) Western blot of mTOR with mitochondria isolated from MCF-7 cells treated with 20 ng/mL rapamycin for 30 min followed by IR. (B) 3D Images of co-localization of TOM40 (green), a mitochondrial outer membrane protein, and mTOR (red) in MCF-7 cells 24 h after treatment with sham, IR or radiation plus rapamycin (IR+Rap). (C) Mitochondrial oxygen consumption, (D) ATP generation, (E) lactate production and (F) clonogenic survival assay at 24 h post-IR in DMSO and rapamycin treated MCF-7 cells. Data are mean ± SEM, p-values were included in each data.